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文献和实验Flow cytometric determination of leukocyte surface antigens in whole blood
by standard venipuncture into an appropriate anticoagulant. Use EDTA or heparin (10 iu/ml). Do not use citrate, this produces an acidic environment which will quench your fluorescein labeled antibody! Collect blood with a large gauge needle into a syringe
protein A-, protein G-, or protein A/G-sepharose and incubate for 30min to 1hr at 4OC with rocking. 10) Collect beads by spinning at either 14000rpm for 10-15” or 5000rpm for 1min (if the latter, spin at 4OC). Carefully aspirate supernatant from beads
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
will have a mother of pearl appearance (not lumpy or black). 4. Spin down agros at 5000rpm for 10 min in 250ml centrifuge bottles, resuspend in infiltration media to an OD600 = 0.8 in a minimum volume of 300ml. 5. Poor the agro suspension into a beaker
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