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文献和实验秀丽线虫RNA提取--C. elegans RNA prep
disposable tube. It should be brownish yellow. 6. You will need to prepare one cesium gradient for each 6 mls of homogenate. Use RNase free 16X102 mm polyallomar tubes, which fit the SW28.1 buckets (which in the Horvitz lab we spin on an SW28 rotor). Don
RNase free 16X102 mm polyallomar tubes, which fit the SW28.1 buckets (which in the Horvitz lab we spin on an SW28 rotor). Don't use " ultraclear" tubes; these crack during the spin. Measure out 11.5 mls of the cesium solution (use a disposable plastic
Using the Generic Genome Browser (GBrowse)
et al., 2006), among others. The software package can be downloaded from the Web and run on a Windows, Mac OS X, or Unix?type system. Version 1.64, as described in the original protocol, was released in November 2005, but the software is under active
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