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上海希言科学仪器有限公司
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文献和实验Isolation of total RNA using CsCl/EtBr gradients--密度梯度法提取总RNA
ethidium bromide, then add 1g of CsCl for each ml of the RNA/EtBr solution, and thoroughly dissolve. 7. Load the solution into a sealable Ti60 tube. Top off the tube with blank CsCl/EtBr sol'n, & seal. Load the tube (and a balance) into the Ti60 rotor
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37?C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells by centrifugation for 20’ at 3,200 RPM in a Sorvall RT7 at 4?C with RTH-250 rotor, ~1700 xg. Decant and drain pellets
(do not let go for more than 14 hrs.) Day Two Spin down cultures in 250 ml bottles in Beckman JA-17 rotor at 6,000 rpm for 15 min. Pour off sups, freeze pellets at -20°C for 10 min.
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