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文献和实验Detection and Measurement of Radioactivity
undergoing 2.22 x 10^12 atomic disintegrations per minute (DPM). A milliCurie (mCi) of a radioisotope undergoes 2.22 x 109 DPM, and a microCurie produces 2.22 x 10^6 DPM. Since 1975, the becquerel (Bq) has replaced the Curie as the preferred international
for 1/8, 1/16, and 1/32 dilutions. Repeat the entire 1/2 dilution series for the 10 dilution. You should now have twelve tubes, six for each of the 10 and the 10 dilutions. These are then diluted 1/2, 1/4, 1/8, 1/16, and 1/32. 4. Use 1.0 ml
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
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