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文献和实验of an ultramicrotome. In principle, it is the offspring of the standard microtome, in that it also is a mechanical device that involves a stationary knife (glass or diamond) and a moving specimen. The specimen, or block, is a plastic embedded tissue that advances
which advances by pre-set intervals with each rotation of the flywheel mounted on the right hand side. In operation, it is similar to the meat and cheese slicers found within delicatessans. A control knob adjusts internal cams which advance the paraffin block
Fluorescence In Situ Hybridization
PRE-TREATMENT Fix the cells with Carnoy’s and drop them onto a glass slide Air dry (and store in -20 ºC until use) Age (if cells are fresh) the cells in 2xSSC, 30 min, 37 ºC Treat with 10-50 µg/mL pepsin in 0.01 N HCl at 37ºC, 1-5
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