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文献和实验Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster
from Grainger rests on top of four 2"x6" boards and one ¾ inch plywood board. The motor, an AC/DC speed control, a fuse, and an on/off switch are wired together into a juncture box. 2) To cool the motor, a hole 3 inches in diameter is cut
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
freezer (polystyrene box wadded with cotton wool [!]). Liquid nitrogen bank. Protocol Spin down cells, remove and discard the supernatant. Resuspend the cells at 5 x 106 per ml in FCS. Add DMSO to 10
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