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文献和实验. We present all relevant methods including the initial site selection, molecular cloning, delivery of guide RNAs (gRNAs) and Cas9 into mammalian cells, verification of target cleavage, and assays for detecting genomic modification including indels
individually in plastic wrap. Take care to remove air bubbles between filter and wrap by pressing with a wipe to force out air. Place two filters in a large cassette (35x43cm), there will be a small overlap of filters but this normally is not a problem
Stereotaxic equipment (e.g., Kopf) Sterotaxic frame 40× microscope attachment
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