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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. A small block made of polyvinyl chloride (2.5 x 2.5 x 1.5 cm) is attached to a flexible Teflon ribbon as shown. The block should hang ~2-3 mm above the plane of the holding block (also made of polyvinyl chloride). A piece of filter paper (i.e., Whatman 3MM
Bromodeoxyuridine Staining of Mammary Tissue
8.5. 8. Rinse 2 x 3 min in PBS. 7. Treat with 0.2% Triton X-100 for 30 minutes at room temperature. 8. Rinse 3 x 5 min in PBS. 9. Block sections 1-2 hours at room temperature with PGB Superblock (see below) in humid chamber. 10. Remove Superblock
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
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