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文献和实验Determining the Direction of Replication Fork Movement
to completion--see The NEB Transcript, Vol. 3 No. 1, March, 1991, pg 8-9.) 1. After staining and photographing the first dimension gel, excise the lane and place it in a disposable pipette reagent reservoir from Costar (#4870; Cambridge MA). These plastic
Determining the Direction of Replication Fork Movement
to completion--see The NEB Transcript, Vol. 3 No. 1, March, 1991, pg 8-9.) 1. After staining and photographing the first dimension gel, excise the lane and place it in a disposable pipette reagent reservoir from Costar (#4870; Cambridge MA). These plastic
Cloning by Limiting Dilution of Hybridoma
and sterile tips Reagent Reservoir HT (Life Technologies, Inc. #11067-030) Procedure The day before the cloning, refeed 24-well plates or flasks with fresh medium. Prepare the cloning medium DMEM 4 mM L-glutamine 20% FBS 10
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