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文献和实验The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques
Primer Design(Manual and Automated Primer Design for SEQ and PHY Gaps)
Length: 18 to 21 nucleotides Tm 56-60℃ 4+2 Rule:Tm = (#G + #C) x 4 + (#A + #T) x 2 Inside the clone Designing PCR Primers for Physical Ends Length: 24 to 28 nucleotides
Marcantonio Lab Protocol Manual
by the following formula: A260 x 12.5 = concentration of DNA (ug/ul) Use the A260/A280 ratio and the A325 to estimate the purity of the nucleic acid sample as follows: Ratios of 1.8 to 1.9 indicate highly purified preparations of DNA. Ratios
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