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文献和实验The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques
Primer Design(Manual and Automated Primer Design for SEQ and PHY Gaps)
Length: 18 to 21 nucleotides Tm 56-60℃ 4+2 Rule:Tm = (#G + #C) x 4 + (#A + #T) x 2 Inside the clone Designing PCR Primers for Physical Ends Length: 24 to 28 nucleotides
Marcantonio Lab Protocol Manual
of cells. Grow overnight at 37 degrees with moderate shaking (200-250 rpm). 2) Inoculate 400 ml of L2 media in a sterile 2 liter flask with 4 ml of the overnight culture. Incubate at 37 degrees with shaking, until an OD590 of 0.375 is achieved
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