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文献和实验ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
scissors and a #3 size scalpel handle Phosphate buffer saline (PBS) Sterile medium size petri dishes (tissue culture standard) 18 gauge needle luer lock syringe (about 6cc should suffice) #11 size flat-edged scaple blade trypsin
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
" (see below) to the oil-free PCR reaction (32µl) into a 1.5ml microtube, vortex briefly to mix and then add 1ml of "Wizard Resin" and vortex briefly, 3 times over a 1 minute period remove the plunger from a 3ml, luer lock syringe and attach the barrel to a clean
equilibration with low salt buffer for 2 hours to increase recovery.) Load a Luer-lock syringe with 5 ml of low salt buffer and attach to minicolumn. Wash matrix by pushing buffer through matrix at approximately 0.5-1.0 ml/min. II. Binding of DNA
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