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文献和实验Transgenic and Knockout Mice PROTOCOL
, and cut the band of interest. 2) Purifiying the DNA fragment. Load about 200-300µg of the gel cut onto a GeneClean spin column(BIO 101'S GeneClean Spinkit)--usually a fragment will require 6-8 columns to take care of all the gel.
1 ul DNA x ul [[gamma]]-[32-P]-rATP 10 uCi T4 polynucleotide kinase 1 ul (3U/ul) 10 ul [[gamma]]-[32-P]-rATP (35020) ICN and T4 polynucleotide kinase (70031) from United States Biochemicals. 2. Incubate at 37degC
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
AJS100-0.5K 4 Thread adaptor, ?-20 male, 8-32 female Newport SS-1-B 2
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