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文献和实验Regulative Development in Axolotl Embryos; Splitting the Heart Field
side facing up, ventral side toward you, and dorsal away. b. In the lower anterior corner there should be a slightly lighter colored region (the presumtive gill tissue); this is what you want to cut out (Figure1); use a tungsten knife to cut and a hair
, add 250 mls fresh NaCl/Tris, rotate 20 minutes.7. Measure gel exactly. Cut off bottom right corner. Put gel back in neutralization solution. Cut nitrocellulose paper about 1 mm shorter than gel and wet in ddH2O. Soak nitrocellulose paper 5-10 minutes
base + 36 g Tris HCl Rotate gently 20 minutes. Decant, add 250 mls fresh NaCl/Tris, rotate 20 minutes. 7. Measure gel exactly. Cut off bottom right corner. Put gel back in neutralization solution. Cut nitrocellulose paper about 1 mm shorter than gel
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