相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
上海希言科学仪器有限公司
- 现货状态:
详询
- 保修期:
详询
- 规格:
详询
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验(like the pRS316 vector) and transforming the MATalpha strain with, for example, any TRP1 plasmid (like the pRS314 plasmid) and then mating the two strains together--only the diploid will grow on the minimal plates. Materials MATa and MATalpha
PCR Protocol for PCR-Mediated Gene Disruption
Procedure 1. Set up the following PCR tube: 5 μl of 10X Taq Buffer 5 μl of 25 mM MgCl2 2 μl of 10 mM dNTPs 10 to 100 ng of template DNA 25 pmol of specific primer 1 25 pmol of specific primer 2 0.5 μl of Taq DNA polymerase (2 Units
Replication timing using transient hemimethylation
and its endogenous promoter were excised from plasmid pMFH1 ( Hoekstra and Malone, 1985 ) with HindIII and PvuII and inserted into the HindIII-SmaI sites of the polylinker in pRS305 (Sikorski and Hieter, 1989), a Leu+ yeast integrating vector. The resulting plasmid
技术资料暂无技术资料 索取技术资料




