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文献和实验Ex vivo enumeration of the absolute frequencies of antigen-specific T cells is key for evaluating the immunogenicity of T cell-based vaccines. Currently there are three methods that are widely used to quantify cellular immune responses
Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
ICs formed by a mixture of autoantibodies and cell debris released from dying cells in culture. To better understand the nature of the mammalian ligands that can effectively activate TLR7 and TLR9, we have developed a methodology for preparing ICs
-3-3 associates with several tyrosine-phosphorylated proteins and phosphatidylinositol 3-kinase (PI3-K) in T cells. We report here the identification of the 120-kDa 14-3-3tau-binding phosphoprotein present in activated T cell lysates as Cbl, a protooncogene
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