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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37?C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells by centrifugation for 20’ at 3,200 RPM in a Sorvall RT7 at 4?C with RTH-250 rotor, ~1700 xg. Decant and drain pellets
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37?C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells by centrifugation for 20’ at 3,200 RPM in a Sorvall RT7 at 4?C with RTH-250 rotor, ~1700 xg. Decant and drain pellets
Lipoprotein Isolation - First week
of a tissue. 14. Place the metal cap of the tubes, and seal with the heat sealer. As soon as the cap reaches the shoulder of the tube, quicly move the tube to the right and push the cap down with the heat sink. 15. Place the four tubes in the VTi
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