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文献和实验. The sensitivity of PCR allows measurement of the expression level of any known gene in a biological sample, in this case, arterial wall tissue. This is achieved by determining the amount of mRNA coding for the target gene product present in the sample. Because PCR
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
. Do not touch the uterus, but ease it back by the edges of the incision in the body wall and allowing the uterus to fall back in, without actually handling it. This procedure is then repeated on the other uterine horn if doing a bilateral transfer. The incision
just inside the open end of the horn. Gently squeeze the plunger on the syringe and flush DMEM with hepes medium through the uterine horn into a sterile petri dish. It is important to flush gently at first otherwise the uterine contents may be lost on a wall, lab coat
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