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文献和实验Analysis of Phospholipids by High Performance Liquid Chromatography
) residues on the glycerol backbone. High performance liquid chromatography (HPLC) has emerged as one of the most powerful and versatile forms of separation technique that can be applied to the efficient separation and determination of phospholipids.
them into their general classes (InsP 1 ; InsP 2 , and so on, and PtdIns, PtdInsP, and PtdInsP 2 ). However the metabolism of phosphoinositols is complex ( 1 , 2 ), leading to the formation of several isomeric forms in each class ( see Fig.1 of Chapter 18 and Note Al
signaling molecules, rendering them inactive. This chapter describes a method that utilizes high-performance liquid chromatography analysis with UV detection for determining the rate of AHL hydrolysis in cell lysates, tissue homogenates, serum
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