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文献和实验.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200
Sample preparation (analytical gels)
(cerebrospinal fluid): An aliquot of 250 μl of human CSF was mixed with 500 μl of ice cold acetone and centrifuged at 10000 g at 4o C for 10 minutes. The pellet was mixed with 10 μl of a solution containing SDS (10% w/v) and DTE (2.3% w/v). The sample was heated
Sample preparation (analytical gels)
urea (8 M),CHAPS (4 % w/v),DTE (65 mM),Tris (40 mM)and a trace of bromophenol blue.Hundred µl of the final diluted colon epithelial cell sample was loaded onto the IPG gel strip. CSF (cerebrospinal fluid): An aliquot of 250 µl of human CSF was mixed
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