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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
, optimize the PCR amplification conditions (i.e., primer concentration, annealing temperature, and cycles). 6) Cloning and Sequencing Note: We highly recommend cloning the PCR products and then performing standard sequencing from at least 96
Determination of Substance Concentration by Competitive ELISA (zt)
of the blocking effect on plates of antigen using an unknown substance with mixtures containing serial dilutions of known amounts of substance and standard concentration of antibodies. It may be helpful to examine the standard protocol for ELISA before performing
An Ultra-fast method of DNA extraction from Neurospora
of 80 isopropanol. Continue aspiration for 2 min to dry out the DE. We use a standard 1 liter filter flask with a #8 stopper drilled with 5 small bore holes. Ten samples can be filtered simultaneously by using two flasks from one aspirator with a Y
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