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文献和实验QuikChange (Stratagene)This is a quick and reliable (and dead easy too!) method for PCR but costs more. It is, however, highly recommended if you need to make only a small number of mutants, or if you can't or won't do any molecular biology
Endothelial Cell Systems - Instructions For Use
each cryovial and add the entire amount to the EBM® with a pipette. Rinse each cryovial with the medium. It may not be possible to recover the entire volume listed for each cryovial. Small losses, even up to 10%, should not affect the cell growth
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
-1 has been combined with the reagents in our LIVE/DEAD Sperm Viability Kit (L7011, Section 15.3) to permit simultaneous assessment of cellular integrity and mitochondrial function by flow cytometry.55 We also offer JC-1 as part of the MitoProbe JC-1 Assay Kit for flow
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