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- 技术资料
- 注册证号:
咨询销售
- 保存条件:
-20度
- 保质期:
6个月
- 英文名:
GQH-HSB
- 库存:
大量
- 供应商:
中乔新舟
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21
种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品
人肝星状细胞生物学定量PCR芯片试剂盒描述:

ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
- Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
- Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
- Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
- Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
- ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
- Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
- Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| A |
ACTA2 |
COL2A1 |
CXCL2 |
FGF1 |
IGF1 |
LAMB1 |
MYH10 |
NGFR |
RARA |
SPARC |
TIMP1 |
β-actin |
| B |
B9D1 |
COL4A2 |
CXCL3 |
FGF2 |
IGF2 |
LAMB3 |
MYH11 |
PDGFA |
RBP1 |
SPP1 |
TMEM67 |
GAPDH |
| C |
B9D2 |
CRABP1 |
CXCL8 |
FOXF1 |
IL1A |
LAMC1 |
MYH9 |
PDGFB |
RBP2 |
STX2 |
TNC |
LDHA |
| D |
BDNF |
CRABP2 |
CYP26A1 |
GFAP |
ITGB3 |
LEP |
NCAM1 |
PDGFC |
RBP4 |
SYNM |
TNF |
NONO |
| E |
CC2D2A |
CSF1 |
CYP26B1 |
HAS1 |
KLF6 |
LHX2 |
NCAM2 |
PDGFD |
RBP5 |
SYP |
VCAM1 |
PPIH |
| F |
CCL2 |
CTGF |
DES |
HGF |
LAMA1 |
LRAT |
NES |
PKD1 |
RELN |
TGFB1 |
VCAN |
GDC |
| G |
CDH1 |
CXCL10 |
EGF |
ICAM1 |
LAMA2 |
MMP13 |
NFKB1 |
PKHD1 |
RPGRIP1L |
TGFBI |
VIM |
PPC |
| H |
CLEC3B |
CXCL12 |
F2 |
IFNG |
LAMA3 |
MMP2 |
NFKB2 |
PTN |
SLC17A5 |
THBS2 |
VTN |
NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK091 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-HSB |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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