For the analysis of low concentrated RNA samples down to 50 pg/µL of total RNA or 250 pg/µL of mRNA. The kit includes 25 microfluidic chips, reagents and consumables.
For the analysis of low concentrated RNA samples down to 50 pg/µL of total RNA or 250 pg/µL of mRNA. The kit includes 25 microfluidic chips, reagents and consumables.
RNA Kits & Reagents - Details & Specifications
RNA quality control and quantitation is essential before any downstream application. Both RNA kits offer:
Quantitation and integrity analysis of total RNA or mRNA
RNA Integrity Number (RIN) – to compare integrity of total RNA samples
Visualization of rRNA impurities in mRNA samples
High sensitivity RNA analysis, e.g. samples derived from microdissection
RNA QC The electropherograms shows the analysis of high quality total RNA (RIN 9.2) in comparison to a partially degraded total RNA (RIN 5.8). (Application Note 5989-1165EN)
Analytical specifications
RNA 6000 Nano Kit
RNA 6000 Pico Kit
total RNA
mRNA
total RNA
mRNA
Quantitative range
25 - 500 ng/µL
25 - 250 ng/µL
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-
Qualititative range
5 - 500 ng/µL
5 - 250 ng/µL
50 - 5000 pg/µL in water
250 - 5000 pg/µL in water
Sensitivity (S/N>3)
5 ng/µL in water
25 ng/µL in water
50 pg/µL in water or 200 pg/µL in TE
250 pg/µL in water or 500 pg/µL in TE
Quantitation reproducibility (within a chip)
10% CV
10% CV
20% CV
20% CV
Quantitation accuracy1
20% CV
20% CV
30% CV
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Maximum sample buffer strength
100 mM Tris 0.1 mM EDTA or 125 mM NaCl 15 mM MgCl2
100 mM Tris 0.1 mM EDTA or 125 mM NaCl 15 mM MgCl2