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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
CD34
- 亚型:
Mouse Ig2b
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
BIOT
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:4H11
- 抗体英文名:
CD34 Antibody-BIOT;流式抗体
- 抗体名:
CD34 Antibody-BIOT;流式抗体
- 规格:
25/100 μg
克隆类型 :Mouse Monoclonal Antibody;Clone number:4H11
货 号 :HT1610885
规 格 :25/100 μg ;
亚 型 :
Mouse Ig2b
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 1.0 µg per 10^6 cells in 100 µl volume or 100 µl of whole blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
BIOT(其他荧光标记请咨询我们)
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- 作者
- 内容
- 询问日期
文献和实验Ex Vivo Production of Cord Blood CD34+Derived Myeloid Precursors After Serum-Free Static Culture
into a closed system. A mean recovery of 90 mL of placental blood is obtained, containing an average of 4 x 106 cells expressing the CD34 antigen. The clinical series published analyzing this transplant modality showed potential advantages in comparison
MACSQuant Tyto 分选有效提高造血干细胞移植与基因修饰
旎基于微芯片技术的 MQ Tyto 细胞分选(Tyto)后细胞的表型 (phenotyping)、基因转导率(GFP expression & vector copy number)、干细胞干性维持(CFC assays)与小鼠模型体内植入 (mouse transplant)。 实验流程与 CD34+CD90+ 细胞评估 1. MQ Tyto 细胞分选后,CD34+CD90+ HSPC 纯度可自 16.3% 提升至 77.5%。产率达 49.9% CD34+CD90+纯度与产率
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
antibody and resuspend in PBS/2% FCS. 10. Separate the CD34+ cells as follows: i. Wash a MACS cell separation column with 200 µL of PBS/2% FCS. ii. Add the labeled cells to the column and place
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