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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD25
- 亚型:
Mouse IgG1
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PE-CY7
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:HI25a
- 抗体英文名:
CD25 Antibody-PE-CY7;流式抗体
- 抗体名:
CD25 Antibody-PE-CY7;流式抗体
- 规格:
25/100 tests
克隆类型 :Mouse Monoclonal Antibody;Clone number:HI25a
货 号 :HT1610849
规 格 :25/100 tests;
亚 型 :
Mouse IgG1
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/ macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used from 20 µL to 5 µL per 100 µL of peripheral blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PE-CY7(其他荧光标记请咨询我们)
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文献和实验条件之一。Reagents for Foxp3 Detection检测Foxp3的试剂 (上海鑫乐生物提供eBioscience公司全系列产品)EBioscience利用蛋白表位图谱构建抗体来检测Foxp3。用Foxp3鉴定Treg细胞通过流式抗体标记CD4+CD25+Foxp3+细胞可以容易的鉴定小鼠脾脏细胞和人PBMC中Treg。一般情况下,CD4+CD25+细胞和Foxp3+细胞相关性接近100%。在特定条件下Foxp3+Treg细胞会下调Foxp3表达,从而丧失免疫抑制功能
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
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Intracellular Immunofluorescent Staining for Flow Cytometry
11): Left: Human peripheral blood cells were stimulated for 2 days with IL-2. The cells were surface stained with FITC CD56 (MEM188) (Cat. No. 11-0569 ) and stained intracellularly with PE GB11. Quadrants demarcate boundary for isotype controls.
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