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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD8
- 亚型:
Mouse IgG1
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PE-CY7
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:HIT18a
- 抗体英文名:
CD8 Antibody-PE-CY7;流式抗体
- 抗体名:
CD8 Antibody-PE-CY7;流式抗体
- 规格:
25/100 tests
克隆类型 :Mouse Monoclonal Antibody;Clone number:HIT18a
货 号 :HT1610723
规 格 :25/100 tests;
亚 型 :
Mouse IgG1
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used from 20 µL to 5 µL per 100 µL of peripheral blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PE-CY7(其他荧光标记请咨询我们)
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文献和实验的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。 不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy
, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-TexasRed, PE-Cy5, PerCP。Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色,但是第4个通道检测荧光素是不一样的 搭配荧光素原则 搭配流式荧光素有2个原则:每个通道只能选择1种荧光素;各个通道之间的荧光素可以随意搭配,但需注意 1、每个通道只能选择1种荧光素。以Calibur为例说明,Calibur
Cell Surface Immunofluorescence Staining Protocol
-AAD with either PE-Cy5 or PE-Cy7 antibody conjugates. 4) Incubate on ice for 3-5 minutes in the dark. 5) Analyze with a Flow Cytometer. 5. Immunofluorescent Staining of Whole Blood: 1) Add predetermined optimum concentrations
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