- ￥7200
- GeneTex
- 美国
- GTX33928-00
- 2025年07月15日
- ELISA, Neutralizing/Inhibition
- Mouse
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Mouse recombinant IL-33.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX33928-00
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ELISA, Neutralizing/Inhibition
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Recognizes mouse IL-33.
- 抗体英文名：
IL33 antibody [Bondy-1-1] (Azide free)
- 抗体名：
IL33 抗体 [Bondy-1-1] (Azide free)
- 规格：
100 μg
Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free). Methods: IL-33 (mouse) was coated on an ELISA plate at 1μg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 100μl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).
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文献和实验Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
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