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Chromeo™ Red Fluorescent Fixed Cell Staining Kit
Chromeo™ Red Fluorescent Fixed Cell Staining Kit
effective fluorescent cell stain for immunofluorescence experiments
The Chromeo™ Red Fluorescent Fixed Cell Staining Kit is based on a proprietary dye that stains the nuclear membrane, mitochondria, fibers and nucleoli in fixed cells. In parallel, the nuclei can be stained with DAPI, which is also included in the kit. The combination of cellular and nuclear staining is ideal for use as a counterstain in immunofluorescence experiments in combination with FITC or other 488 nm excitable fluorescent dyes like Chromeo™ 488. In addition, the kit can be used to monitor changes in the localization and shape of nuclei, to highlight changes in cellular morphology and to monitor cell-cell contacts within a population of cells. The kit is suitable for use in both fluorescence microscopy and high-content screening (HCS).
Contents & Storage
1 vial of lyphilized Chromeo™ Red Fluorescent Fixed Cell Stain (store at -20°C in the dark)
1 vial DMF (store at 4°C)
2 vials MAXfluor™ DAPI Mounting Medium (store at 4°C in the dark)
Contents guaranteed 6 months when stored properly.
The kit is designed to stain 260 slides based on the use of 22 mm square coverslips, or 2500 wells in 8-well chambered slides, or 5000 wells in a 96-well plate. The number of experiments will depend on the chosen concentration of the stain, which may vary for different cell types. The dyes included in the Chromeo™ Red Fluorescent Fixed Cell Staining Kit can be excited with commonly used excitation sources and standard filter sets of fluorescent microscopes or readers. The cellular stain can be excited between 520 nm and 550 nm (max at 540 nm) and emits at 627 nm. This long Stokes shift separates the emission spectrum of the cellular stain from the emission spectra of GFP or 488 nm excitable dyes, which enables co-staining experiments. The broad emission spectrum of the stain allows flexibility and optimal intensity in detection. The DAPI nuclear stain has its excitation maximum at 358 nm and emission maximum at 461 nm. DAPI filter sets are available in most fluorescence microscopes and fluorescent plate readers.
The dyes included in the Chromeo™ Red Fluorescent Fixed Cell Staining Kit can be excited with commonly used excitation sources and standard filter sets of fluorescent microscopes or readers. The cellular stain can be excited between 520 nm and 550 nm (max at 540 nm) and emits at 627 nm. This long Stokes shift separates the emission spectrum of the cellular stain from the emission spectra of GFP or 488 nm excitable dyes, which enables co-staining experiments. The broad emission spectrum of the stain allows flexibility and optimal intensity in detection. The DAPI nuclear stain has its excitation maximum at 358 nm and emission maximum at 461 nm. DAPI filter sets are available in most fluorescence microscopes and fluorescent plate readers.