红色荧光固定细胞染色试剂盒

Contents & Storage

• 1 vial of lyphilized Chromeo™ Red Fluorescent Fixed Cell Stain (store at -20°C in the dark)
• 1 vial DMF (store at 4°C)
• 2 vials MAXfluor™ DAPI Mounting Medium (store at 4°C in the dark)

Contents guaranteed 6 months when stored properly.

The kit is designed to stain 260 slides based on the use of 22 mm square coverslips, or 2500 wells in 8-well chambered slides, or 5000 wells in a 96-well plate. The number of experiments will depend on the chosen concentration of the stain, which may vary for different cell types.
The dyes included in the Chromeo™ Red Fluorescent Fixed Cell Staining Kit can be excited with commonly used excitation sources and standard filter sets of fluorescent microscopes or readers. The cellular stain can be excited between 520 nm and 550 nm (max at 540 nm) and emits at 627 nm. This long Stokes shift separates the emission spectrum of the cellular stain from the emission spectra of GFP or 488 nm excitable dyes, which enables co-staining experiments. The broad emission spectrum of the stain allows flexibility and optimal intensity in detection. The DAPI nuclear stain has its excitation maximum at 358 nm and emission maximum at 461 nm. DAPI filter sets are available in most fluorescence microscopes and fluorescent plate readers.

The dyes included in the Chromeo™ Red Fluorescent Fixed Cell Staining Kit can be excited with commonly used excitation sources and standard filter sets of fluorescent microscopes or readers. The cellular stain can be excited between 520 nm and 550 nm (max at 540 nm) and emits at 627 nm. This long Stokes shift separates the emission spectrum of the cellular stain from the emission spectra of GFP or 488 nm excitable dyes, which enables co-staining experiments. The broad emission spectrum of the stain allows flexibility and optimal intensity in detection. The DAPI nuclear stain has its excitation maximum at 358 nm and emission maximum at 461 nm. DAPI filter sets are available in most fluorescence microscopes and fluorescent plate readers.