生物活性
X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].
体外研究
(In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc Dicyclohexylamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
分子量
603.93
性状
Solid
Formula
C26H36BrClN2O7
CAS 号
18656-96-7
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:
DMSO : ≥ 30 mg/mL (49.67 mM)
* "≥" means soluble, but saturation unknown.
配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 1.6558 mL 8.2791 mL 16.5582 mL
5 mM 0.3312 mL 1.6558 mL 3.3116 mL
10 mM 0.1656 mL 0.8279 mL 1.6558 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: