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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
IL-4
- 亚型:
Rat IgG1
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:11B11
- 抗体英文名:
IL-4 Antibody-FITC;流式抗体
- 抗体名:
IL-4 Antibody-FITC;流式抗体
- 规格:
50/500 μg
克隆类型 :Rat Monoclonal Antibody;Clone number:11B11
货 号 :HT1610471
规 格 :50/500 μg ;
亚 型 :
Rat IgG1
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
FITC(其他荧光标记请咨询我们)
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文献和实验Dual- and Triple-Color Fluorospot
in several publications and reagents/kits for various cytokine combinations and species are commercially available. The most common protocol for fluorospot includes the use of biotinylated detection antibodies for one cytokine and an FITC-labeled detection antibody
Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
Intracellular Immunofluorescent Staining for Flow Cytometry
Brefeldin A BVD6-24G2 , 11B11 IL-5 NEW! mouse splenic CD4 ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hr
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