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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD16/CD32 (FcγRII/III)
- 亚型:
Rat IgG2b
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
BIOT
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:2.4G2
- 抗体英文名:
CD16/CD32 (FcγRII/III) Antibody-BIOT;流式抗体
- 抗体名:
CD16/CD32 (FcγRII/III) Antibody-BIOT;流式抗体
- 规格:
50/500 μg
克隆类型 :Rat Monoclonal Antibody;Clone number:2.4G2
货 号 :HT1610133
规 格 :50/500 μg ;
亚 型 :
Rat IgG2b
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII and FcγII receptors. It has also been reported to bind the FcγI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, receptors in vitro and in vivo. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 0.25 µg/10^6 cells in 100 µl
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
BIOT(其他荧光标记请咨询我们)
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文献和实验到的细胞类型表达FcR,同时反应抗体含有Fc段,都应该考虑进行Fc 阻断,来确保实验的特异性,减少非特异的产生。 那么,具体哪些细胞类型需要做Fc阻断? 首先,按FcR所结合的Ig种类不同可将其分为五类,即IgG(FcγR)、IgE(FcεR)、IgA(FcαR)、IgM(FcμR)和IgD(FcδR)。 IgG类型抗体的Fc受体是FcγR,依据和Fc片段亲和力的强弱,FcγR又可以被分为三个亚家族:FcγRI(CD64)、FcγRII(CD32)和FcγRIII(CD16)以及进一步还可以分为不同
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
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