手机验证
充足
杭州昊鑫生物
7.8-500pg/mL
双抗夹心
详见说明
Sus scrofa; Porcine (Pig,猪)
详见说明
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
48T
本试剂盒用于检测白介素12B(IL12B),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的白介素12B(IL12B)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 95-103 | 101 |
EDTA plasma(n=5) | 88-102 | 95 |
heparin plasma(n=5) | 80-96 | 86 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的白介素12B(IL12B),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素12B(IL12B)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-104% | 97-105% | 85-105% | 78-103% |
EDTA plasma(n=5) | 85-92% | 86-101% | 91-105% | 97-105% |
heparin plasma(n=5) | 96-104% | 88-98% | 84-95% | 98-105% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将白介素12B(IL12B)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的白介素12B(IL12B)与连接于固相载体上的抗体结合,然后加入生物素化的白介素12B(IL12B)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素12B(IL12B)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
杂志 | 参考文献 |
Anticancer Research | Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ. [Pubmed: 23564769 ] |
Scandinavian Journal of Immunology | Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. [pubmed:27384769] |
Journal of Functional Foods | Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis [S1756464616303528] |
ACS Biomaterials-Science & Engineering | Photothermal-triggered Controlled Drug Release From Mesoporous Silica nanoparticles Based On Base-pairing Rules [] |
Antibiotics | Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs [Pubmed: 31963653] |
Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … [Pubmed: 32680553] |
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杂志 | 参考文献 |
Anticancer Research | Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ. [Pubmed: 23564769 ] |
Scandinavian Journal of Immunology | Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. [pubmed:27384769] |
Journal of Functional Foods | Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis [S1756464616303528] |
ACS Biomaterials-Science & Engineering | Photothermal-triggered Controlled Drug Release From Mesoporous Silica nanoparticles Based On Base-pairing Rules [] |
Antibiotics | Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs [Pubmed: 31963653] |
Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … [Pubmed: 32680553] |