干扰素α(IFNa)真核蛋白

干扰素α(IFNa)真核蛋白

Purity≥ 93%；Fragment：Cys24~Leu181； Predicted Molecular Mass(KD)：19.8kDa

优尔生的一级代理：杭州昊鑫生物

优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,003种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。

• 编号EPA033Hu61
• 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
• 来源真核表达
• 宿主293F cell
• 内毒素水平<1.0EU per 1ug (determined by the LAL method)
• 亚细胞定位分泌
• 预测分子量19.8kDa
• 实际分子量20kDa(差异分析请参阅说明书)
• 片段与标签Cys24~Leu181 with N-terminal His Tag
• 缓冲液成份20mM Tris, 150mM NaCl缓冲液，（pH8.0,含有1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300）
• 性状冻干粉
• 纯度> 95%
• 等电点4.9
• 应用Positive Control; Immunogen; SDS-PAGE; WB.
  如果需要有生物活性的蛋白，请参见活性蛋白。
• 下载英文说明书 中文说明书
• 规格10µg50µg 200µg 1mg 5mg

产品包装图模拟

用法

PBS或其它。

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

参考文献

杂志	参考文献
Journal of Animal and Veterinary Advances	Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616]
Journal of Animal and Veterinary Advances	Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600]
Ann Rheum Dis.	The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats [PubMed: 23172753]
Nanomedicine	Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132]
International journal of experimental pathology	Experimental rat model for alcohol-induced osteonecrosis of the femoral head [PubMed: PMC3781777]
Antiviral Research	A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus [ScienceDirect: S0166354214000266]
British Journal of Pharmacology	Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells [Onlinelibrary: bph.12440]
BMC Cancer	High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model [Pubmed:24950740]
PLoS One.	Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs [Pubmed:25036811]
Veterinary Research	Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750]
Asian Pac J Trop Med	Effect of SecinH3 on lung injury induced by sepsis of rats [PubMed: 26706678]
PLoS One	siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455]
Plos One	Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway. [Pubmed:27023634]
PLoS One.	Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. [Pubmed:23776655]
Veterinary microbiology	Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). [pubmed:27599928]
International Journal of Clinical and Experimental Pathology	Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf]
Scandinavian Journal of Immunology	Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. [pubmed:27384769]
Saudi Journal of Gastroenterology	Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice. [pubmed:27976641]
Biochemical and Biophysical Research Communications	MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861]
Virology	The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058]
Antiviral Research	Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease [pubmed:28454913]
Molecular Therapy. Methods & Clinical Development	Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice [pubmed:28649578]
Journal of Virology	Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. [pubmed:28915824]
Clinical Immunology	Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis [Pubmed:30056130]
International forum of allergy & rhinology	Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis [Pubmed:29901280]
LUPUS	Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754]
Journal of Pineal Research	Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267]
Veterinary Immunology and Immunopathology	Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens []
international immunopharmacology	Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813]
FASEB Journal	Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 []
Transboundary and Emerging Diseases	Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856]
archives of virology	A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets [Pubmed: 32253616]
Autophagy	LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761]
THERIOGENOLOGY	Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion [Pubmed: 32810792]
Int Immunopharmacol	BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566]
Molecular Medicine Reports	Aquaporin‑4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978]
Virulence	Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon … []
РОССИЙСКАЯ СЕЛЬСКОХОЗЯЙСТВЕННАЯ НАУКА	ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ []
Communications Biology	Influenza virus neuraminidase regulates host CD8+ T-cell response in mice [33293641]
Animals	Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants [33494350]
Int J Dermatol	IFN©\¦Á and TNF©\¦Á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata [34008204]
Dig Dis Sci	Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway [34097168]
	Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-¦Ã immunodeficiency: a prospective cohort study [34144700]
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY	Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats [34555716]
Carbohydrate Polymers	The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides [34823813]