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- 文献和实验
- 技术资料
- 保存条件:
详见说明
- 保质期:
详见说明
- 英文名:
Recombinant Elastin (ELN)
- 库存:
充足
- 供应商:
杭州昊鑫生物
- 规格:
10ug
弹性蛋白(ELN)重组蛋白
Recombinant Elastin (ELN)
优尔生的一级代理:杭州昊鑫生物
优尔生位于武汉出口加工区,已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证,以提供生命科学科研用检测试剂为主,75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存,能够做到约11,000种蛋白、19,000种抗体以及73115种检测试剂盒立等可取。蛋白项目部拥有四大技术平台,包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核(E.Coli)表达系统与真母、杆状病毒-昆虫细胞、哺乳动物细胞)表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制,主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制,包括原料检测、半成品检测及成品检测。
- 编号RPB337Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌, 细胞外基质
- 预测分子量22.6kDa
- 实际分子量28kDa(差异分析请参阅说明书)
- 片段与标签Gly392~Ala645 with N-terminal His Tag
- 缓冲液成份100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点10.3
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg 200µg 1mg 5mg
- 价格¥ 1008
序列
用法
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
参考文献
| 杂志 | 参考文献 |
| Clinical & Experimental Immunology | Induction of elastin expression in vascular endothelial cells relates to hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum anti-endothelial cell antibodies [PubMed: 22288597] |
| Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. [Pubmed: 24739658] |
| Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Nutrition & Diabetes | Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling. [pubmed:27941938] |
| FREE RADICAL BIOLOGY AND MEDICINE | Anti-ageing effects of Ubiquinone and Ubiquinol in a senescence model of human dermal fibroblasts [33482334] |
| GENE THERAPY DNA VECTOR VTvaf17, METHOD OF PRODUCTION; ESCHERICHIA COLI STRAIN SCS110-AF, METHOD OF PRODUCTION; ESCHERICHIA?¡ [] | |
| ARCHIV?EUROMEDICA | CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE [] |
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文献和实验参考文献
| 杂志 | 参考文献 |
| Clinical & Experimental Immunology | Induction of elastin expression in vascular endothelial cells relates to hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum anti-endothelial cell antibodies [PubMed: 22288597] |
| Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. [Pubmed: 24739658] |
| Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Nutrition & Diabetes | Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling. [pubmed:27941938] |
| FREE RADICAL BIOLOGY AND MEDICINE | Anti-ageing effects of Ubiquinone and Ubiquinol in a senescence model of human dermal fibroblasts [33482334] |
| GENE THERAPY DNA VECTOR VTvaf17, METHOD OF PRODUCTION; ESCHERICHIA COLI STRAIN SCS110-AF, METHOD OF PRODUCTION; ESCHERICHIA?¡ [] | |
| ARCHIV?EUROMEDICA | CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE [] |
【整理】蛋白版06年9月份无人应助帖,请广大战友积极应助,加分从优!
蛋白酶和弹性蛋白酶的关系 http://www.dxy.cn/bbs/post/view?bid=65&id=7051240&sty=1&tpg=15&age=0 【求助】关于转基因植物蛋白纯化的问题,急求赐教 http://www.dxy.cn/bbs/post/view?bid=65&id=7050078&sty=1&tpg=15&age=0 【求助】硝化蛋白质的检测 http://www.dxy.cn/bbs/post/view?bid=65&id=7049959&sty
有关。但是对这一多肽与ureC编码的多肽均未明确其作用。从DNA序列图谱上看,表明这些多肽对把尿素酶活性转移给空肠弯曲菌受体是必需的。 ②细胞毒素相关基因CagA和空泡毒素VacA:自从发现Hp高分子量抗原与消化性溃疡病有关后,引起了人们对它的基因的兴趣。Tumuru等用λZapII构建了Hp84-183的随机染色体片段文库。在大肠杆菌YLi-Blue的多个文库中用Hp感染者经过吸收的血清筛选。结果分离纯化出一个3.5kb插入片段的克隆。这一插入片段的再克隆能表达出一种Hp的重组蛋白
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