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- Roche
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- 11745832910
- 2025年07月15日
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- 文献和实验
- 技术资料
- 保存条件：
详见说明
- 库存：
详询
- 供应商：
上海古朵生物科技有限公司
- 规格：
25 g
Features and Benefits
Sigma Life Science is committed to bringing you greener alternative products, which adhere to one or more of the 12 principles of greener chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG system, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Other Notes
仅用于生命科学研究。不可用于诊断。
包装
1 kit containing 7 components.
Quality
Function tested in a dot blot.
Application
DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime-labeled DNA probes are used in a variety of hybridization techniques:
• Southern blots[3][4]
• Northern blots
• Dot blots[2]
• Colony and plaque hybridizations
• For all types of filter hybridization
• For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat
General description
The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand.
This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT (nitroblue tetrazolium)/BCIP (5-bromo-4-chloro-3-indol
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文献和实验标记DNA探针 如果您有足够量的高纯度模板，建议您采用随机引物标记方法的DIG High Prime DNA Labeling and Detection Starter Kit I 和II。这两个试剂盒包含了标记、封闭、杂交和检测所需要的所有试剂，是真正意义上的一站式服务的试剂盒。二者之间的差别在于检测方法的不同：Starter Kit
【建议】Southern和Northern中的奇怪问题？请大家讨论。
的DIG Northern Starter Kit，没有用他的显色底物，而是用的DIG High Prime DNA Labeling and Detection Starter Kit I中的NBT/BCIP底物，因为我没有显影液和定影液，所以没有用CDP-Star。还有为什么在边缘的条带跑的快，是不是MOPS电泳缓冲液的原因，我在整个电泳过程中没有换过缓冲液，电泳大约2个小时左右，没40分钟停止电泳，拿出胶后搅一搅缓冲液。 第二张图是Southern杂交，用的是DIG High Prime
地高辛（ DIG ）产品选择指南 ―― 选择合适的标记方法 标记DNA 探针 如果您有足够量的高纯度模板，建议您采用随机引物标记方法的 DIG High Prime DNA Labeling and Detection Starter Kit I 和 II 。这两个试剂盒包含了标记、封闭、杂交和 检测 所需要的所有试剂，是真正意义上的一站式服务的试剂盒。二者之间的差别在于检测方法的不同： Starter Kit I 采用了显色法，使用 显色
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