EB proteins belong to the microtubule plus end tracking family proteins. They interact with cytoplasmic microtubules in interphase cells, with mitotic spindles, and with the adenomatous polyposis coli (APC) tumor suppressor. The end-binding protein 1, 2 and 3 (EB1-3) are involved in microtubule polymerization and stabilization with centrosomes as well as spindle function. The N-terminal domain of EB proteins binds to microtubules whereas the C-terminal domain forms mutually exclusive complexes with APC and DCTN1. This antibody recognizes the human, monkey, mouse and hamster EB1 in ELISA, Western blot, immunoprecipitation and immunofluorescent staining.
克隆类型:
Rat Monoclonal Antibody
亚型:
rat IgG2a
免疫原:
Recombinant human EB1[125-268]-GST fusion protein
适用物种:
H, M, Mk, other
应用范围:
E, WB, IP, IF
应用说明:
WB: 1:2,000; IF: 1:200
别名:
Microtubule-associated protein RP/EB family member 1; End-binding protein 1; EB1; APC-binding protein EB1; MAPRE1
形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
保存条件:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.