- 询价
- ATCC
- 美国
- ATCC CCL-70 CV-1
- 2025年10月07日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北纳创联
- 细胞形态：
fibroblast
| 基本信息 | |
| 资源编号 | ATCC CCL-70 |
| 资源名称 | CV-1 |
| 种属 | Cercopithecus aethiops kidney |
| 形态 | fibroblast |
| 提供形式 | P4-5代T25细胞培养瓶 |
| 致病类型 | Normal |
| 安全等级 | 1 |
| 模式菌株 | 未知 |
| 应用领域 | efficacy testing transfection host viruscide testing |
| 说明书 | 点击下载 |
| 培养方法 | |
| 培养基 | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 传代方法 | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Place culture vessels in incubators at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week |
| 生长条件 | Temperature: 37°C |
| 生长特性 | adherent |
| 存储条件 | liquid nitrogen vapor phase;Culture medium, 95%; DMSO, 5% |
| 详细说明 | |
| Age | 141 days |
| Gender | male |
| Karyotype | This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell. |
| Derivation | The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies. |
| Clinical Data | adult African green monkey male |
| Virus Susceptibility | Human poliovirus 1 Herpes simplex virus Eastern equine encephalitis virus , Eastern equine encephalitis virus Western equine encephalitis virus , Western equine encephalitis virus California encephalitis virus , Melao virus SV40 virus |
| Year of Origin | 1964 |
| Cross References | Nucleotide (GenBank) U83324 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 1, complete cds. Nucleotide (GenBank) U83325 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 2, complete cds. |
| 描述 | This cell line is a suitable host for transfection, especially by SV40 vectors. |
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文献和实验/LGCAdvancedCatalogueSearch/tabid/961/Default.aspx?CollectionID=106 ATCC® Number Description Designation CCL-218 Homo sapiens (human) WiDr CCL-220 Homo sapiens (human) COLO 320DM CCL-220.1 Homo sapiens (human) COLO 320HSR [COLO 320 HSR] CCL-221 Homo
% FBSCRFK CCL-94 猫 肾 上皮细胞、贴壁 MEM/NEAA, 10% FBSCV-1 CCL-70 猴 非洲绿猴肾 成纤维细胞、贴壁 MEM/NEAA, 10% FBS细胞株名称 ATCC 编号 细胞来源 细胞种类 生长状态 使用培养基 D-17 CCL-183 狗 骨肉瘤 上皮细胞、贴壁 MEM/NEAA, 10% FBS DAN CRL-2130 狗 骨肉瘤 上皮细胞、贴壁 MEM/EBSS, 8% FBS Daudi CCL-213 人 淋巴瘤病人血液 成淋巴细胞、悬浮 RPMI
CCL-1) 3.5 None 2 40 7 10 40-150 EMCV @1x104 TNFa L929 (ATCC CCL-1) 3.5 None 2 24
