We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.
规格:
5ML
C57BL/6-GFP Mouse Gingival Epithelial Cells from Creative Bioarray are isolated from C57BL/6-GFP-Tg(CAG-EGFP)1Osb/J mouse gingival tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Gingival Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended. Product Link: http://www.creative-bioarray.com/C57BL-6-GFP-Mouse-Gingival-Epithelial-Cells-CSC-C9095J-item-41520.htm