NIH3T3 are useful for DNA transfection and transformation studies; tested and found negative for ectromelia virus (mousepox). Fetal calf serum is no suitable. The serum initially employed and found to be satisfactory was from the Colorado Serum CompanyTumorigenecity:Isoenzyme:Histopathology:Subculture:Split ratio:Media change:Reverse transcritase:Production Product Link:http://www.creative-bioarray.com/NIH3T3-CSC-C9563L-item-39860.htm