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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Salmonella enterica OmpA family protein (A673_03341), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Salmonella enterica OmpA family protein (A673_03341), partial品名简称:
Recombinant Salmonella enterica OmpA family protein (A673_03341) protein, partial货号:
CSB-MP028238SBG规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
/Uniprot No.:
S4JJH7别名:
/种属:
Salmonella enterica subsp. enterica serovar Enteritidis str. 2009K0958蛋白长度:
Partial来源:
Mammalian cell分子量:
18.6 kDa表达区域:
82-220aa氨基酸序列:
DVQEAKLRDKMRGTGVSVTRSGDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVVGYTDSTGSHDLNMRLSQQRADSVASSLITQGVDASRIRTSGMGPANPIASNSTAEGKAQNRRVEITLSPLQ蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
McClelland M., Porwollik S., Desai P., Cheng P., Wollam A., Pepin K., Palsikar V.B., Fulton L., Fulton R., Delehaunty K., Fronick C., Godfrey J., Waligorski J., Appelbaum E., Tomlinson C., Warren W., Sodergren E., Weinstock G., Wilson R.K.Submitted (APR-2013)研究领域:
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文献和实验质芯片检测。此外,肽芯片或基于质谱的方法[ 20,21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白(MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片(见第 28 章)。在放射性【 γ33 磷】三磷酸腺苷存在的条件下,用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号,对可能
高表达常常导致包涵体的形成,虽然包涵体具有富集目标蛋白质、抗蛋白酶、对宿主毒性小等优点,但包涵体蛋白质的复性率一般都很低,而分子伴侣、低分子量添加物等在复性过程中的应用及新的复性方法的建立都大大提高了重组蛋白质复性产率。 一、包涵体: 1.1包涵体的定义、组成与特性: 包涵体是指细菌表达的蛋白在细胞内凝集,形成无活性的固体颗粒。 一般含有50%以上的重组蛋白,其余为核糖体元件、RNA聚合酶、内毒素、外膜蛋白ompC、ompF和ompA等,环状或缺口的质粒DNA,以及脂体
, small molecules. Key words inclusion body , protein , renaturation 外源基因在大肠杆菌中的高表达常常导致包涵体的形成,虽然包涵体具有富集目标蛋白质、抗蛋白酶、对宿主毒性小等优点,但包涵体蛋白质的复性率一般都很低 , 而分子伴侣、低分子量添加物等在复性过程中的应用及新的复性方法的建立都大大提高了重组蛋白质复性产率。
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