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- 文献和实验
- 技术资料
- 保存条件:
-20
- 英文名:
20-HETE Lipid Maps MS Standard
- 规格:
100 µg
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文献和实验the separated lipid in the -20 °C freezer until transmethylation (day 2). Results You should have obtained a good separation of the lipid classes, similar to the plate shown below. However, differences in the solvent composition
Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
; with a split ratio of 1:20, only 5 % of the volume injected actually enters the column, while 95 % are diverted. For the exact quantitation with the GLC profile and separate standards, it would be necessary to known what proportion of your total lipid
that are reduced to lipid alcohol, such as hydroxyeicosatetraenoic acid (HETEs), and isoprostanes (IsoPs) respectively. The IsoPs are isomers of prostaglandins that are generated from autoxidation of arachidonic acid (C20:4). Quantification of F2 -IsoPs
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