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- 文献和实验
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- 英文名:
Glycerol Stock Solution (20% v/v)
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1 L
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文献和实验Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
) glycerol, 5% (v/v)b-mercaptoethanol, and 0.003% (w/v) bromophenol blue. 2.2. Sodium Dodecyl Sulfate (SDS) Polyacrylamide Gel Electrophoresis (PAGE) 1. Separating buffer (4×): 1.5 M Tris-HCl, pH 8.7, and 0.4%(w/v) SDS. Store at room temperature (RT
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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