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- 文献和实验
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- 规格：
100 assays
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文献和实验DAY 1 1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance from 480 to 0.4 ng/ml
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
ul of each of the above dilutions to 25ul of 3x strength titre of antibody (i.e. a solution 3 x the titre giving 50% inhibition on direct ELISA), in sealed tubes. 3. Incubate overnight at room temperature. 4. Set up competition between unknown concentration of competitive
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