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文献和实验Serum Creatine Kinase in Progressive Muscular Dystrophies
Several enzymes originating in muscle show increased activity in the serum of patients affected with different forms of muscular dystrophy (MD). These enzymes include creatine kinase (CK), aldolase, transaminase, and pyruvate kinase (PK
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
in transglycosylation reaction. In the assay, UDP was coupled to the conversion of phosphoenolpyruvate to pyruvate using pyruvate kinase. Using a commercial pyruvate assay kit, the pyruvate was converted to a red terminal product, which could be photometrically measured
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
. Caspase enzymatic assay After incubation of the extracts in the presence or absence of activating stimuli (cytochrome c/dATP or active recombinant caspase), caspase enzymatic activity was measured
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