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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
ZOOM® IPGRunner™系统:简化的双向电泳(2D gel eletrophoresis)
V 电泳45分钟。 染色 按照标准程序,使用SilverQuest™银染试剂盒(SilverQuest™ Silver Staining kit )( Invitrogen, Cat. no. LC6070)或SimplyBlue™ SafeStain (Invitrogen, Cat. no. LC6060)对2D胶进行染色。 结果和讨论 图2 是按照图
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
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