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文献和实验Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity
S -adenosylmethionine decarboxylase (AdoMetDC; EC 4.1 1.50) catalyzes the conversion of S -adenosylmethionine to S -5′-deoxyadenosyl-(5′)-3-methylthiopropylamine (decarboxylated S-adenosylmethionine) and CO2 . The enzyme is unusual
Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine
L -Ornithine decarboxylase (EC 4.1.1.17) (ODC) catalyzes the conversion of L -ornithine to putrescine and CO2 . ODC is dependent on pyridoxal 5′-phosphate (PLP) and thiol-reducing agents for activity (1 ). At least two key active site
Determination of Ornithine Decarboxylase Activity Using [3H]Omithine
: assay of ODC mRNA; assay of ODC protein; and assay of ODC activity.
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