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- 规格：
100 assays
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文献和实验) to check derivatisation and chromatography efficiency and to allow quantitation of samples. Note that the Fmoc-amino acid derivatives are stable for 24 h, allowing many samples to be prepared in advance and loaded onto an autosampler for injection
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
of amino acids can bind with Cu 2+ to form a complex in the basic solution and then the Cu 2+ is deoxidized to Cu + . Bicinchoninic acid and its sodium salt are water-soluble. In the alkaline environment, it can bind with Cu
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