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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
LIVE/DEAD® Violet Viability/Vitality Kit
. Material Amount Storage Stability CellTrace™ calcein violet stain, lyophilized (Component A) *
Alanine Transaminase Activity Assay
0, 2, 4, 6, 8, 10 nmol/well of the Pyruvate Standard for the colorimetric assay. 2) Fluorometric assay: Dilute the Pyruvate Standard to 1 nmol/µl as for the colorimetric assay. Then dilute the standard another 10-fold to 0.1 nmol/µl by taking 10
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