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文献和实验complex from step 10 above. Vortex briefly to mix and incubate at room temperature for 15 minutes with rotation. Spin down agarose, and carefully transfer the supernatant fraction (eluate) to another tube and repeat elution. Combine eluates (total
Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue
weight tissue), and sub-types from kidney tissues is therefore an important part of determining the extent of renal fibrosis in ageing and diseased states. This chapter describes a colorimetric-based hydroxyproline assay used to estimate total collagen
Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants
of radioactivity from cartilage explants previously labeled with 35 SO4 2− . Collagen degradation is calculated as percent release of collagen, measured by colorimetric assay of hydroxyproline.
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